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BIOCHEMICAL EFFECTS OF SULPHUR DIOXIDE ON PROLINE METABOLISM IN THE ALGA TREBOUXIA SP.
Author(s) -
EWALD D.,
SCHLEE D.
Publication year - 1983
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1983.tb04496.x
Subject(s) - proline , metabolism , sulfur metabolism , chemistry , botany , biology , biochemistry , amino acid
SUMMARY Cells of the green alga Trebouxia sp. treated with Na 2 SO 3 (5 × 10 −5 to 5 × 10 −3 M) for 4, 24, 48, and 72 h show a decreased content of proline and an increase in alanine dependent on incubation time and sulphite concentration. The activity of the NAD‐dependent proline dehydrogenase (EC 1.5.1.2, alternative name, pyrroline‐5‐carboxylate reductase) was measured. In vitro sulphite uncompetetively inhibited the enzyme. In vivo , sulphite concentrations up to 5 × 10 −4 M led to enhanced enzyme activity. It is suggested that sulphite influences the proline content in Trebouxia via the last enzyme of proline synthesis, proline dehydrogenase.