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EFFECT OF ETHYLENE ON GIBBERELLIC ACID‐I NDUCED PROTEASE ACTIVITY IN WHEAT ALEURONE LAYERS *
Author(s) -
VARTY K.,
ARREGUIN L. B.,
MÜGGENBURG R MA. I.,
MIJANGOS M. J. L.,
TREJO B. A.
Publication year - 1983
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1983.tb04494.x
Subject(s) - gibberellic acid , aleurone , protease , ethylene , biochemistry , enzyme , biology , gibberellin , proteolytic enzymes , plant hormone , chemistry , botany , gene , germination , catalysis
SUMMARY Ethylene‐stimulated gibberellic acid‐induced protease synthesis in isolated wheat aleurone layers ( Triticum aestivum L.) by about 60%. Ethylene did not change the isoelectric focusing pattern of protease isoenzymes, indicating that the hormone promoted only quantitative changes in protease synthesis rather than activating genes for different proteolytic enzymes. The pH optimum and K m of the enzyme with respect to N,N' ‐dimethylhaemoglobin were 3.5 and 0.102% respectively. The purified protease consisted of a single polypeptide with a molecular weight of 43 700 daltons. These physical characteristics of gibberellic acid‐induced protease were unaffected by ethylene.