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NITROGEN METABOLISM IN HALOPHYTES V. THE OCCURRENCE OF MULTIPLE FORMS OF GLUTAMINE SYNTHETASE IN LEAF TISSUE
Author(s) -
McNALLY SHEILA F.,
HIREL B.,
STEWART G. R.
Publication year - 1983
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1983.tb02720.x
Subject(s) - gene isoform , glutamine synthetase , isozyme , biochemistry , glutamine , biology , cytoplasm , ion chromatography , enzyme , botany , amino acid , gene
S ummary Ion‐exchange chromatography was used to examine the occurrence of glutamine synthetase (GS) isoforms in leaf tissue of 18 species of coastal plants. Two groups of species were recognizable on the basis of their complement of GS isoforms. One group comprised those species which exhibited two forms of GS, the properties of which were consistent with their being cytoplasmic and chloroplastic isoforms. In the second group were species which had only a single isoform, and exhibited properties similar to those of the chloroplastic isoform. Immunoprecipitation studies with antibody raised to the cytoplasmic isoenzyme of barley were carried out to identify the isoform present in species having only one peak of activity on ion‐exchange chromatography. No relationship was evident between either total GS activity or the occurrence of the cytoplasmic isoform and the photo respiratory capacity of different species.