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A STUDY OF THE DEVELOPMENT OF RAPHIDE‐FORMING CELLS IN MUSA PARADISIACA USING FLUORESCENCE MICROSCOPY *
Author(s) -
BRUNI ALESSANDRO,
DALL'OLIO GIUSEPPE,
TOSI BARBARA
Publication year - 1982
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1982.tb03417.x
Subject(s) - musa × paradisiaca , calcium oxalate , cell wall , fluorescence microscope , biophysics , root hair , polysaccharide , crystallization , oxalate , fluorescence , cytoplasm , chemistry , morphogenesis , microbiology and biotechnology , botany , biology , biochemistry , gene , physics , organic chemistry , quantum mechanics
SUMMARY The development of raphide‐forming cells and the differentiation of calcium oxalate needles were investigated in young banana stems using fluorescence microscopy. By employing highly sensitive procedures for the detection of carbohydrates, proteins and RNA, six functional stages in raphide‐cell ontogeny were defined: (1) the appression of cytoplasm to the cell wall in young idioblasts; (2) the synthesis of a polysaccharide matrix with early lacunae; (3) the formation in the lacunae of chambers, the envelope of which contains RNA and protein; (4) the synthesis of glycoproteins and polysaccharides in the chamber envelopes; (5) initiation of the formation of closely packed raphide bundles and the beginning of the crystallization of calcium oxalate and (6) new crystals are formed in a polysaccharide lattice produced in the mucilaginous bundle sheath. The results presented are compared with those of other investigations on the pattern of idioblast development and calcium oxalate deposition in higher plants.