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ORIGIN AND EARLY GROWTH OF CELERY EMBRYOIDS
Author(s) -
DUNSTAN D. I.,
SHORT K. C.,
MERRICK M. A.,
COLLIN H. A.
Publication year - 1982
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1982.tb03297.x
Subject(s) - cotyledon , suspension (topology) , suspension culture , agar , botany , embryoid body , biology , callus , chemistry , biophysics , cell culture , in vitro , biochemistry , bacteria , genetics , pure mathematics , endothelial stem cell , mathematics , adult stem cell , homotopy
SUMMARY Suspension cultures of celery embryoids were initiated by transferring portions of differentiating callus to liquid medium. Light microscope studies of the cells and embryogenic aggregates in the suspensions suggested that the embryoids arose from either single cells within the embryogenic aggregates, when the developing embryoid remained attached to the clump until the globular stage, or from free floating small groups of cells. Both types of development showed the celery embryoids to be of single cell origin. When the embryoid suspension was filtered to remove the larger embryoids and the small embryogenic aggregates and free floating embryoids were mixed with agar and plated, the development of the embryoids depended on the initial inoculum density. At low density further embryoid development was delayed while at the high inoculum density the embryoids became green and grew rapidly into torpedo embryoids with cotyledon leaves and roots. At this stage they could be transferred to a simple inorganic medium to develop into plantlets. The effect of inoculum density was thought to be due to a conditioning effect of the environment. It is suggested that the differentiating units in the embryoid suspension could be used in a cell selection procedure.