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A STUDY OF THE INTRACELLULAR AND INTERCELLULAR DISTRIBUTION OF THE DATURA STRAMONIUM LECTIN USING AN IMMUNOFLUORESCENT TECHNIQUE
Author(s) -
JEFFREE C. E.,
YEOMAN M. M.
Publication year - 1981
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1981.tb03218.x
Subject(s) - datura stramonium , lectin , intracellular , phloem , biology , microbiology and biotechnology , plant cell , endodermis , protoplast , organelle , botany , biochemistry , gene
SUMMARY The intracellular and intercellular distribution of the lectin in seeds, stem and callus of Datura stramonium was visualized by means of an indirect immunofluorescent technique employing antibodies, raised in a rabbit against highly purified lectin obtained from Datura seeds. The lectin was detected in the protoplasts of all cells tested, apparently associated with the plasmalemma and the membranes of cell organelles. Cell walls appear to be free of lectin. In stems, where lectin concentrations are very low, epidermis, endodermis and phloem are the richest sources. The significance of the distribution of the D. stramonium lectin is discussed in relation to the possible functions of this molecule.