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THE ACID INVERTASES OF THE DEVELOPING THIRD LEAF OF OAT
Author(s) -
GREENLAND A. J.,
LEWIS D. H.
Publication year - 1981
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1981.tb01724.x
Subject(s) - invertase , isozyme , size exclusion chromatography , ion chromatography , biology , enzyme , biochemistry , chromatography , chemistry , botany
SUMMARY Soluble acid invertases, obtained from the developing third leaf of oat, were fractionated by gel filtration and ion‐exchange chromatography. Gel filtration separated two peaks. Isoenzyme II (85 × 10 3 daltons) was the dominant form in the non‐emerged leaf but decreased in activity after leaf emergence. The activity of isoenzyme I (143 × 10 3 daltons) remained constant throughout leaf development and was dominant in mature tissues. Ion‐exchange chromatography separated two discrete peaks (A and B) and a composite peak (C), comprised several isoenzymes. The activities associated with A, B and C varied considerably during leaf development. One or more of the isoenzymes of invertase in peak C was the predominant invertase at all stages of leaf development. These observations are discussed in relation to the role and control of synthesis of invertases during development.