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THE EFFECT OF l ‐ARGININE ON THE GROWTH OF HETEROTROPHIC CULTURES OF THE EMERSON STRAIN OF CHLORELLA . III. STUDIES WITH THE STRUCTURAL ANALOGUE CANAVANINE
Author(s) -
Thinh L. V.,
Griffiths D. J.
Publication year - 1980
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1980.tb04550.x
Subject(s) - canavanine , cell division , arginine , chlorella , dna synthesis , biochemistry , biology , cell growth , microbiology and biotechnology , dna replication , protein biosynthesis , strain (injury) , dna , cell , algae , botany , amino acid , anatomy
S ummary The special role of arginine in controlling cell division in heterotrophic cultures of Chlorella emersonii has been studied using the guanidoxy structural analogue, canavanine. Evidence is presented that competition between the two compounds probably occurs at the site of protein synthesis and that the analogue interferes with the synthesis of proteins essential for cell division and chloroplast development. An experimental system based on the light or dark recovery of previously heterotrophically grown cells has shown that protein synthesis which takes place after DNA replication is particularly sensitive to canavanine, and that the initial stages of recovery are important in countering the inhibitory effect of the analogue. The possibility is discussed that arginine‐proteins may have a general role in controlling cell division in Chlorella emersonii .