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DISTRIBUTION OF ATPASE IN CELLS OF SALICORNIA PACIFIC A VAR. UTAHENSIS AS DETERMINED BY LEAD PHOSPHATE PRECIPITATION AND X‐RAY MICROANALYSIS
Author(s) -
WEBER D.J.,
HESS W. M.,
KIM CHONG KYUN
Publication year - 1980
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1980.tb04428.x
Subject(s) - atpase , microanalysis , cytoplasm , chemistry , phosphate , biophysics , ouabain , membrane , cortex (anatomy) , palisade cell , cytochemistry , ultrastructure , biochemistry , biology , botany , sodium , enzyme , organic chemistry , neuroscience
SUMMARY Salicornia pacifica stem tissues were treated with ATP, Ca(NO 3 ) 2 and Pb(NO 3 ) 2 to localize ATPase. ATPase activity was present along the plasma membranes and in the cytoplasm of the cortical palisade, inner cortex and vascular cells of young shoots. ATPase was also present in the plasma membranes of tracheids which are present in the palisade region. Ouabain did not inhibit ATPase, but NaF‐, crown ethers, and N.N′‐dicyclohexyl carbodiimide (DCCD) did so in vitro. Only DCCD significantly inhibited (82 %) ATPase in tissues processed for electron microscopy. Energy dispersive microanalysis was used to verify that the precipitates were Pb phosphate.