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THE PERIHAUSTORIAL MEMBRANE IN HOP CELLS INFECTED BY PSEUDOPERONOSPORA HUMULI
Author(s) -
Pares R. D.,
Greenwood A. D.
Publication year - 1979
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1979.tb07472.x
Subject(s) - haustorium , phosphotungstic acid , uranyl acetate , membrane , staining , chemistry , biology , botany , biochemistry , host (biology) , ecology , genetics , catalysis
S ummary The part of the host plasmalemma invaginated around the haustorium of Pseudoperonospora humuli was examined after staining with (a) Uranyl acetate followed by lead citrate and (b) a mixture of phosphotungstic acid and chromic acid. It was also examined by using a tilting and rotating goniometer stage. After staining with uranyl acetate and lead citrate the membrane could only be detected on about 45 % of the periphery of the haustorium transected in a section and only the outer dark component of the triplet unit membrane structure could be detected. The use of phosphotungstic acid‐chromic acid enabled the whole of the triplet membrane structure to be seen but did not enable the membrane to be detected over a greater percentage of the haustorium periphery than with the first procedure. The use of the goniometer stage resulted in the membrane being seen around 95 % of the periphery of the haustorium.