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THE USE OF SEED ISOENZYMES AS AN AID TO THE BREEDING OF FIELD BEANS ( VICIA FABA L.)
Author(s) -
GATES P.,
BOULTER D.
Publication year - 1979
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1979.tb02309.x
Subject(s) - vicia faba , isozyme , esterase , biology , cotyledon , genotype , outcrossing , botany , horticulture , biochemistry , enzyme , gene , pollen
S ummary A rapid and economical method has been developed for identifying inbred lines and detecting crossing between them. It employs non‐specific esterase and glutamate‐oxaloacetate transaminase isoenzyme patterns of cotyledon extracts, separated by electrophoresis on acrylamide gels. Isoenzyme patterns were genotype‐specific and stable from plants grown in a range of environmental conditions. The potential value of these isoenzymes as markers for genotype identification and measurement of outcrossing is illustrated and discussed in relation to the breeding of field beans.