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THE EFFECT OF SIMAZINE ON THE GROWTH AND RESPIRATION OF A CELL SUSPENSION CULTURE OF CELERY
Author(s) -
METCALF E. C.,
COLLIN H. A.
Publication year - 1978
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1978.tb02630.x
Subject(s) - simazine , respiration , respiratory rate , respiration rate , zoology , biology , chemistry , botany , horticulture , agronomy , pesticide , atrazine , endocrinology , heart rate , blood pressure
SUMMARY Celery cell suspension cultures, inoculated into liquid medium containing simazine and maintained for 3 weeks, showed little growth and low respiratory activity at concentrations of 1.0 and 10.0 mg 1 −1 simazine but growth and respiration were normal with 0.01 and 0.1 mg 1 −1 simazine. The addition of 1.0 or 10.0 mg 1 −1 simazine to a cell culture caused an immediate stimulation of respiration rate; lower concentrations of simazine were ineffective. The initial high respiratory rate in 10 mg 1 −1 simazine was not maintained and after 6 h the rate had declined to the level of the control. When the cells were incubated in 10 mg 1 −1 simazine for a longer period the rate declined to zero after 1 week, cell division was inhibited and the dry weight decreased. The initial high rate of respiration in simazine, followed by a decline, combined with the inhibition of growth and finally cell death suggested that the simazine may be acting as a respiratory uncoupler.