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PREPARATION OF PLANT MITOCHONDRIA USING GEL FILTRATION COLUMNS
Author(s) -
HARLEY J. L.,
BLACK M. K.,
WEDDING R. T.
Publication year - 1978
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1978.tb02628.x
Subject(s) - centrifugation , sephadex , chromatography , size exclusion chromatography , chemistry , filtration (mathematics) , dilution , differential centrifugation , biochemistry , enzyme , statistics , mathematics , thermodynamics , physics
SUMMARY The possibility of preparing plant mitochondria using Sephadex or Bio‐gel columns was investigated. It is concluded that gel filtration is a feasible alternative to centrifugation with many plant materials. It has the advantage of providing preparations of higher O 2 uptake rate per unit protein with similar or better ADP/O or respiratory control ratios than centrifugation. It is often also more rapid for routine preparation. Its disadvantage in particular is the dilution of the final suspension.