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INHIBITION BY ZINC OF SOLUBLE AND CELL WALL ACID PHOSPHATASES OF ZINC‐TOLERANT AND NON‐TOLERANT CLONES OF ANTHOXANTHUM ODORATUM
Author(s) -
COX R. M.,
THURMAN D. A.
Publication year - 1978
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1978.tb02260.x
Subject(s) - zinc , biology , enzyme , phosphatase , biochemistry , cell wall , botany , chemistry , organic chemistry
SUMMARY Inhibitor constants, K i and K' i for zinc have been determined for a number of soluble and cell‐wall‐bound acid phosphatases of two zinc‐tolerant and two non‐tolerant clones of Anthnxanthum odoratum. The values of K i for the soluble enzymes of both clones approached infinity which suggested that the inhibition was mainly of an uncompetitive nature. The tolerant clones possessed K i values for soluble and cell wall enzymes which were significantly higher than the corresponding values for the non‐tolerant clones. K' i values of the cell wall enzymes of tolerant and non‐tolerant clones were not significantly different from one another. It was concluded that non‐tolerant clones possess acid phosphatases which form more stable complexes with zinc than tolerant ones. The relevance of this observation to metal tolerance mechanisms is discussed.