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STUDIES ON THE GROWTH OF THE ENDOPHYTE OF OLALNUS GLUTINOSA (L.) VILL. IN NUTRIENT SOLUTIONS
Author(s) -
QUISPEL A.,
TAK T.
Publication year - 1978
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1978.tb01632.x
Subject(s) - hypha , axenic , spore , inoculation , biology , incubation , nutrient , botany , infectivity , chemistry , petroleum ether , microbiology and biotechnology , chromatography , biochemistry , bacteria , horticulture , ecology , genetics , extraction (chemistry) , virus , virology
SUMMARY During incubation of nutrient solutions heavily inoculated with an axenic suspension of spore‐nodules of Alnus glutinosa (L.) Vill. we observed an increase in infective capacity. This effect was only obtained with nutrient solutions to which an extract with petrol ether‐soluble substances from roots had been added. The reproducibility of the experiments is definitely increased when the active lipid factor(s) is further purified by silicagel column chromatography. Aerobic conditions are essential. Microscopic examination of the infective nutrient solutions showed the presence of Actinomycete‐like hyphae. These hyphae were never observed in controls where the lipid extracts had been omitted. After several transfers to fresh media, cultures are still highly infective and microscopically characterized by growth of these hyphae. After inoculation with axenic suspensions of spore + nodules, neither an increase of infectivity nor growth of hyphae was observed.