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CARBOHYDRATE METABOLISM IN AGARICUS BISPORUS (LANGE) IMBACH.: METABOLISM OF [ 14 C] LABELLED SUGARS BY SPOROPHORES AND MYCELIUM
Author(s) -
HAMMOND J. B. W.,
NICHOLS R.
Publication year - 1977
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1977.tb02211.x
Subject(s) - trehalose , mannitol , fructose , biochemistry , agaricus bisporus , mycelium , metabolism , carbohydrate metabolism , carbohydrate , chemistry , biology , botany , mushroom
SUMMARY When growing sporophores and vegetative mycelium of Agahcus bisporus had been supplied with [ 14 C] labelled hexoses for varying periods of time, the major compounds labelled in both forms were mannitol, trehalose, glutamate, alanine and an unidentified substance. Mannitol was strongly labelled after application of [ 14 C] fructose but only weakly when [ 14 C] glucose was supplied. The relative rates of oxidation by sporophores of labelled mannitol, trehalose, glucose and fructose were assessed by measuring 14 CO 2 production. Glucose and trehalose were the most rapidly oxidized substrates. Glucosephosphatase isomerase (E.C. 5.3.1.9) was extracted from sporophores and assayed. The conversion of glucose‐6‐phosphate to fructose‐6‐phosphate by the enzyme was competitively inhibited in vitro by 6‐phosphogluconate. The control of mannitol synthesis and the functions of mannitol and trehalose are discussed.