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STUDIES ON INSECT‐FREE GROWTH, DEVELOPMENT AND NITRATE‐ASSIMILATING ENZYMES OF DROSERA ALICIAE HAMET *
Author(s) -
SMALL G. C.,
ONRAËT ANGELA,
GRIERSON D. S.,
REYNOLDS GAYNOR
Publication year - 1977
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1977.tb02188.x
Subject(s) - nitrate reductase , glutamate synthase , glutamate dehydrogenase , glutamine synthetase , nitrite reductase , nitrate , ammonium , peroxidase , enzyme , chemistry , botany , nitrite , nitrogen , biology , biochemistry , glutamine , glutamate receptor , organic chemistry , amino acid , receptor
Summary The carnivorous plant, Drosera aliciae , cultivated insect‐free from seed showed better growth with nitrogen as NH 4 Cl and NH 4 NO 3 than with NaNO 3 . Highest yields of NaNO 3 ‐treated plants were obtained at pH 4.0 and 4.5 whereas with NH 4 Cl and NH 4 NO 3 yields increased with increasing pH. Plants grown on a nitrogen‐free medium but fed cheese were little affected by pH. Plants flowered and produced viable seeds in all treatments. The enzymes, nitrate reductase, nitrite reductase, a peroxidase capable of reducing nitrate, gluta‐mate dehydrogenase, glutamate synthase and glutamine synthetase were demonstrated in cell‐free extracts of roots and leaves. Enzyme levels in ammonium, cheese and nitrate‐fed plants were compared.