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DENSITY LABELLING DURING MICROBODY DEVELOPMENT IN COTYLEDONS
Author(s) -
BROWN RICHARD H.,
MERRETT MICHAEL J.
Publication year - 1977
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1977.tb02182.x
Subject(s) - glyoxysome , microbody , peroxisome , cucurbita pepo , isocitrate lyase , biochemistry , biology , darkness , cotyledon , cytochemistry , differential centrifugation , botany , glyoxylate cycle , enzyme , gene
Summary During the development of pumpkin (Cucurbita pepo) cotyledons peroxisomal development was dissociated completely from glyoxysomal decline by keeping seedlings in darkness for up to 20 days; glycollate oxidase activity resulting from illumination then occurred after complete decay of isocitrate lyase. During cotyledon development a peak of phosphoryl‐choline glyceride transferase activity preceded maximal development of glyoxysomes while a second peak of activity occurred after illumination but before maximal development of peroxisomes. When dark‐grown cotyledons were illuminated on transfer to 2 H 2 O and micro‐bodies isolated by gradient centrifugation, peroxisomes were separated from glyoxysomes because of their greater equilibrium density. The observed density shift for peroxisomes suggests that they arise by de novo synthesis rather than by modification of glyoxysomes.