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THE EFFECT OF L‐ARGININE ON THE GROWTH OF HETEROTROPHIC CULTURES OF THE EMERSON STRAIN OF CHLORELLA
Author(s) -
THINH L. V.,
GRIFFITHS D. J.
Publication year - 1977
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1977.tb01550.x
Subject(s) - arginine , chloramphenicol , biochemistry , chlorella , biology , dna synthesis , nucleic acid , biosynthesis , heterotroph , strain (injury) , metabolism , chloroplast , amino acid , bacteria , algae , dna , antibiotics , botany , enzyme , genetics , anatomy , gene
SUMMARY The ability of L‐arginine to stimulate autospore production in heterotrophic cultures of the Emerson strain of Chlorella is reversed by chloramphenicol. The antibiotic does not affect the uptake of glucose, neither does it alter significantly the pattern of incorporation of radioactivity from 14 C glucose into the major fractions of arginine‐treated cultures. The inhibitory effect of the antibiotic is largely restricted to those aspects of development which specifically respond to arginine. Thus, the stimulation of DNA synthesis by arginine is strongly reversed by chloramphenicol suggesting the involvement of some chloroplast‐centered event. Chloramphenicol does not inhibit the uptake of arginine but affects the subsequent metabolism of the amino acid. It is concluded that addition of arginine may lead to the synthesis in the chloroplast of specific polypeptides necessary for nucleic acid synthesis and cell division.