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ENDOPOLYPLOIDY IN MAIZE SMUT NEOPLASMS INDUCED BY THE MAIZE SMUT Fungus, USTILAGO MAYDIS
Author(s) -
CALLOW J. A.
Publication year - 1975
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1975.tb01394.x
Subject(s) - ustilago , biology , feulgen stain , smut , meristem , botany , nucleolus , mitosis , ploidy , fungus , dna , microbiology and biotechnology , nucleus , genetics , shoot , gene
SUMMARY Infection of maize seedlings by Ustilago maydis results in neoplasia. The response of host nuclei and nucleoli to infection in these neoplasms has been examined in detail by Feulgen microspectrophotometry and quantitative interferometry. Seven days after infection, two distinct tissue zones could be recognized within the neoplastic leaf sheaths. Small cells around the vascular stele contained nuclei of 2C and 4C DNA content suggesting a meristematic function. Larger, vacuolate cells that form the bulk of the tissue contained nuclei of 16C, 32C, and 64C DNA levels, indicating substantial endopolyploidy. Nucleolar dry mass was substantially increased in infected cells (up to ten‐fold compared with controls), indicating enhanced ribosome synthesis. These results are discussed in relation to the hormonal control of DNA synthesis and mitosis.