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ENHANCEMENT OF NITRATE REDUCTASE ACTIVITY IN SENESCING KALE LEAF DISCS BY N,N ‐DIMETHYLAMINO‐SUCCINAMIC ACID
Author(s) -
KNYPL J. S.
Publication year - 1973
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1973.tb02108.x
Subject(s) - nitrate reductase , cycloheximide , nitrate , chemistry , brassica oleracea , chloramphenicol , brassica , botany , reductase , horticulture , enzyme , biochemistry , biology , protein biosynthesis , antibiotics , organic chemistry
SUMMARY Freshly cut discs (385 mm 2 total area) from mature leaves of kale (Brassica oleracea L. var. acephala) produced about 70 nmol NO ‐ 2 when incubated with 0.02 M KNO 3 for 3 hours. The amount of NO ‐ 2 formed in the 3‐hour test was smaller when the discs were incubated on water for 24 hours before the test but it was much increased when the discs were incubated for 24 hours with KNO 3 , KNO 3 plus N,N ‐dimethylamino‐succinamic acid (B‐Nine) or B‐Nine alone. The effort of B‐Nine on increasing nitrate reductase activity was abolished by cycloheximide and unaffected by chloramphenicol. It is concluded that B‐Nine can induce the synthesis of nitrate reductase in the absence of light or of nitrate.