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IDENTIFICATION OF ABSCISIC ACID IN YOUNG STEMS OF PINUS RADIATA D. DON
Author(s) -
JENKINS P. A.,
SHEPHERD K. R.
Publication year - 1972
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1972.tb01950.x
Subject(s) - chemistry , abscisic acid , chromatography , pinus radiata , fluorescence , radiata , mass spectrum , gas chromatography , mass spectrometry , ether , electrophoresis , biochemistry , botany , biology , organic chemistry , physics , vigna , quantum mechanics , gene
S ummary An inhibitor from Pinus radiata was separated using paper chromatography from an acidic ether fraction of a stem tissue extract. Using electrophoresis the inhibitory activity was resolved into two parts. One part remained at the origin. The second part behaved similarly to ABA, in electrophoresis and was investigated further. The inhibitor has similar properties to ABA in electrophoresis; TLC using a number of solvent systems; UV fluorescence spectra: UV absorption; ORD spectra and gas chromatography. Final proof of the identity of the natural inhibitor as ABA was obtained by gas chromatography‐mass spectrometry.