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THE CYTOKININS OF CULTURED SYCAMORE CELLS
Author(s) -
MACKENZIE I. A.,
STREET H. E.
Publication year - 1972
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1972.tb01272.x
Subject(s) - cytokinin , zeatin , callus , botany , biology , auxin , chemistry , biochemistry , gene
S ummary Two cytokinin fractions, active in the tobacco and soybean callus bio‐assays, have been obtained from sycamore cells cultured in a cytokinin‐free medium. One of these (Cyt 1) corresponds in its properties to an uncharacterized cytokinin detected in water melon and cucumber seeds. The second fraction (Cyt 2) is coincident with zeatin riboside in chromatographic properties. No evidence was obtained for the presence of a ‘nicotinamide‐like’ cytokinin. The ‘staled’ culture medium which had supported the growth of sycamore cells contained Cyt 1 activity, low Cyt 2 activity and a cytokinin coincident with zeatin in chromatographic mobility. There is a rapid rise in the cytokinin activity in the cells during the lag phase which precedes active division in batch culture. Later the cytokinin activity per cell and per unit volume of culture progressively declines.