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MORPHOGENETIC STUDIES ON CULTURED MATURE ENDOSPERM OF CROTON BONPLANDIANUM
Author(s) -
BHOJWANI S. S.,
JOHRI B. M.
Publication year - 1971
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1971.tb02576.x
Subject(s) - endosperm , kinetin , callus , biology , botany , embryo , croton , yeast , tissue culture , microbiology and biotechnology , genetics , in vitro
S ummary The mature endosperm of Croton bonplandianum proliferated profusely on White's medium (WM) + 2 ppm 2,4‐D + 5 ppm kinetin + 2500 ppm yeast extract. Association with the embryo was essential for initiation of proliferation of mature endosperm. Tracheidal cells usually differentiated. Twelve‐month‐old callus contained predominantly triploid and hexaploid cells. When the callus obtained on WM + 2,4‐D + kinetin + yeast extract was subcultured on WM triploid roots formed in 25% of the cultures. At 10 −7 M, IBA, IAA, or NAA, in WM, promoted rooting; IBA was most effective. Attempts to induce shoot differentiation failed.