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QUINONES AND α‐TOCOPHEROL IN GREENING CALLUS CULTURES OF KALANCHOË CRENATA
Author(s) -
THOMAS D. R.,
STOBART A. K.
Publication year - 1971
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1971.tb02521.x
Subject(s) - callus , greening , chloroplast , plastoquinone , botany , chlorophyll , biology , photosynthesis , chemistry , biochemistry , thylakoid , ecology , gene
S ummary Dark‐grown Kalanchoë callus tissue subcultured into 16‐hour days produce chlorophyll only after several generations. Plastoquinone: chlorophyll molar ratios in extracts from green callus indicate that the level of plastoquinone is somewhat lower than in leaf tissue. This was confirmed in purified chloroplast preparations from callus and leaves. Hill reaction activity was lower in green callus chloroplasts than in those from mature leaves. α‐Tocopherol, although present in non‐green callus, increased with chlorophyll synthesis. D, L[2 14 C]‐mevalonate feeding experiments seem to indicate active sites of α‐tocopherol synthesis other than the mature chloroplast. Changes in ubiquinone and α‐tocopherol content were observed during the growth of dark‐grown callus. Such changes may be due to an increase in mitochondrial populations and to the onset of senescence.