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AUTORADIOGRAPHIC LOCALIZATION OF SOLUBLE AND INSOLUBLE 14 C FROM [ 14 C]INDOLYLACETIC ACID SUPPLIED TO ISOLATED COLEUS INTERNODES
Author(s) -
WANGERMANN ELISABETH
Publication year - 1970
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1970.tb02471.x
Subject(s) - plant stem , phloem , chemistry , xylem , pith , leaching (pedology) , botany , nuclear chemistry , biology , ecology , soil water
S ummary Methods for localizing soluble label in Plant tissues using frozen sections and stripping film are discussed. After 24 hours' application, 14 C derived from radioactive indolylacetic acid (IAA) is heavily concentrated in the phloem, less so in the xylem, and very weak in the cortex and pith. The 14 C cannot be ‘chased’ out of the tissues by non‐radioactive IAA, but nearly all of it is removed by leaching sections in water or methanol. The small amount which remains after leaching is attached to the walls of vessel elements. Chromatograms of extracts of treated internodes show that very little of the label seen in the tissues remains attached to IAA, and that most of it is present associated with substances which remain near the origin.