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NITROGEN METABOLISM IN DEVELOPING SEEDS OF VICIA FABA
Author(s) -
BOULTER D.,
DAVIS O. J.
Publication year - 1968
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1968.tb06405.x
Subject(s) - vicia faba , point of delivery , globulin , storage protein , ovule , biology , nitrogen , botany , polyacrylamide gel electrophoresis , protein metabolism , amino acid , albumin , ontogeny , chemistry , biochemistry , enzyme , pollen , organic chemistry , immunology , gene , genetics
S ummary Fruits of Vicia faba L. were harvested from plants grown outdoors to provide a developmental series trom flower opening to maturity. Pod and seed were separated, extracted, and the changes in distribution of protein and non‐protein nitrogen determined. Amino acid content of pod and seed was determined and changes in albumin protein were characterized by acrylamide gel electrophoresis. The bulk of nitrogenous material accumulated by the seed was found to be globulin protein, and accumulation was found to coincide with the appearance of protein granules in the developing cotyledons. Similar protein bodies were observed in the maternal tissues of the young ovule, but not in pod tissues, and possible sources of nitrogenous material destined for globulin synthesis are discussed.