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SOME EFFECTS OF ADENINE ON GROWTH, RESPIRATION, ADENOSINE DIPHOSPHATE AND TRIPHOSPHATE CONCENTRATIONS IN PLANT TISSUES
Author(s) -
MERRETT M. J.,
HANDOLL C.
Publication year - 1967
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1967.tb05429.x
Subject(s) - respiration , purine , biochemistry , callus , adenine nucleotide , adenosine triphosphate , dinitrophenol , nicotiana tabacum , respiration rate , biology , adenosine , chemistry , nucleotide , botany , enzyme , gene
S ummary Conditioned callus tissues of Nicotiana tabacum type White Burley variety Judy's Pride were grown on basal media supplemented with purine or pyrimidine bases. A substantial increase in ATP concentration in the tissues occurred only with adenine. The accumulation of ATP was not the result of growth inhibition and the ATP concentration of the tissues was increased by adenine concentrations which did not inhibit growth. When exogenous adenine was added to tissues grown on basal media, the respiration rate was not increased. The respiration rate of tissues grown on adenine‐containing media was the same as that of tissues grown on basal media. Although adenine increased the total amount of ATP in the tissue, the ADP concentration was unatfected. Dinitrophenol stimulated the respiration rate and this, taken together with the unaltered ADP concentration, suggests that the availability of phosphate acceptors still controls the respiration rate of tissues grown with adenine in the medium. The response of tobacco callus tissue to adenine infected with tobacco mosaic virus was similar to that of non‐infected tissue.