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CELL WALL METABOLISM I. HEMICELLULASES OF BROMUS SEEDS
Author(s) -
MACLEOD ANNA M.,
SANDIE R.
Publication year - 1961
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1961.tb06245.x
Subject(s) - cellulose , cellulase , germination , husk , cellobiose , bromus , botany , chemistry , cell wall , enzyme , biology , biochemistry , poaceae
S ummary Readily extractable hemicelluloses account for approximately 14% of the dry weight of grains of five species of Bromus . In B. mollis , although 10% of cellulose is present in the whole grain, only one‐eighth of this cellulose is located within the true seed, the remainder being restricted to the husk. Crude preparations of enzymes extracted from B. mollis can rapidly solubilize and degrade pentosan and hexosan hemicelluloses but are without apparent effect on suspensions of cellulose, and there appears to be no real evidence for the action of a true cellulase during germination. Extracts of Bromus seeds are also extremely active in transglucosylation and can readily catalyse synthesis of β‐linked oligosaccharides from 1% solutions of cellobiose.