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CYTOGHROME OXIDASE IN MYCORRHIZAL AND UNINFEGTED ROOTS OF FAGUS SYLVATICA
Author(s) -
HARLEY J. L.,
PREES T. A.
Publication year - 1959
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/j.1469-8137.1959.tb05368.x
Subject(s) - cyanide , cytochrome c oxidase , azide , chemistry , biochemistry , carbon monoxide , cytochrome , oxidase test , electron transport chain , alternative oxidase , respiration , malonate , enzyme , biology , botany , organic chemistry , catalysis
S ummary The effect of azide, cyanide, carbon monoxide and DNP upon the respiration of mycor‐rhizal roots of beech is stimulatory. The stimulations are shown to be the result of the reaction of the fungal sheath. Host tissue of mycorrhizas and of uninfected roots react in a more expected fashion and are inhibited by azide, cyanide and carbon monoxide. In spite of this the fungal sheath is shown to possess a succinic dehydrogenase‐cytochrome oxidase system inhibited by malonate, cyanide and azide. Host tissue, whether from infected or uninfected roots, gives extracts containing a heat‐stable reductant of cytochrome C which interferes with spectrophotometric assay of the oxidase system. It is concluded that a significant part of electron transport of intact mycorrhizas may pass through the cytochrome oxidase system and, on inhibitor evidence alone, that the respiration of the host tissues is catalysed also by such a system. An explanation of stimulatory effects of inhibitors of cytochrome oxidase is suggested and discussed.