Ultrastructure of Holothuria polii encapsulating body

The coelomic cavity of freshly collected Holothuria polii specimens contains a variable number of brown pigmented and unpigmented encapsulating structures. They are composed of nodules with entrapped parasites and an internodular mass which comprises a number of nodules. Both brown and unpigmented bodies occur in different size classes depending upon the number of nodules accumulated in a complete body. The unpigmented bodies probably represent an early unmelanized stage of the brown ones. The nodule was ultrastructurally constituted by foreign bodies surrounded by a fibrous, electron‐dense, non‐cellular layer, probably melanin, followed by a layer of elongated and extremely flattened amoebocytes. Nodules were assembled in an internodular mass formed by amoebocytes type I, II and III spherule cells. As for the amoebocytes constituting the nodule, those present in the spaces between nodules did not develop junctional complexes. It is conceivable that the intricate network established among the cell processes could represent the mechanical force maintaining the whole structure. As suggested by our results, two functional amoebocyte populations seem to be responsible for the organization of the scavenger body: 1. encapsulating amoebocytes, characteristically non phagocytosing, elongated cells; and 2, phagocytosing amoebocytes. The former organize the nodules, the latter constitute the internodular mass of several nodules. Most probably, the double scavenger activity justifies the considerable complexity of the H. polii encapsulating structure compared to other invertebrates. Spherule cells participate only in constituting the internodular mass.