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Hormone induced chromatophore changes in the European tree frog, Hyla arborea, in vitro
Author(s) -
Nielsen Hans Ingolf,
BereiterHahn Jürgen
Publication year - 1982
Publication title -
journal of zoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.915
H-Index - 96
eISSN - 1469-7998
pISSN - 0952-8369
DOI - 10.1111/j.1469-7998.1982.tb02082.x
Subject(s) - chromatophore , melanophore , biology , tree frog , contraction (grammar) , medicine , endocrinology , in vitro , melatonin , melanocyte stimulating hormone , hormone , melanosome , zoology , biochemistry , melanin , fishery
The colours of the European tree frog, Hvlu urhorea , depend on three types of chromatophores: in dermo‐epidermal direction melanophores, iridophores, and xanthophores. The ability ofthis species to assume a wide range ofcolours implies that very extensive changes in the chromatophores take place, which in turn require control by several regulating factors. The responses of the different chromatophore types to hormones with known melanophore‐affecting abilities (α‐MSH, β‐MSH, ACTH, melatonin) were tested in an in vitro system (freshly explanted skin) using reflectance microspectrophotometry, light microscopy and time‐lapse cinemicrography. α‐MSH, β‐MSH and ACTH all induce a rapid dispersion of melanosomes during the 10 min after addition. The degree of pigment dispersion induced by ACTH is slightly less than after stimulation with α‐MSH or β‐MSH. The iridophores react to MSH or ACTH treatment with a contraction of the entire cell (causing a reduction in reflecting area), and a change in orientation of the platelets, causing a decrease in selective reflectance. The iridophores appear to be especially sensitive to ACTH. A very striking feature of the iridophores when studied with time‐lapse cinematography is their strong pulsations (approx. once per minute). The xanthophores react to MSH and ACTH with a contraction. These cells appear to be sensitive to β‐MSH in particular. Melatonin strongly counteracts the effects of α‐MSH, β‐MSH and ACTH on all chromatophores. These studies confirm the dynamic nature not only of the melanophores, but also of the iridophores and xanthophores, as pointed out by Schmidt (1920) and Nielsen (1978a). Furthermore the differences in the time course of the stimulation of the different types of chromatophores by various hormones may provide an experimental basis for the explanation of colour changes in Hyfa arboreu.