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Renal Physiology
Author(s) -
ANDREW COSTIN
Publication year - 2003
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2003.tb00611.x
Subject(s) - citation , computer science , information retrieval , library science
The conventional model of renal glucose reabsorption involves uptake from the proximal tubule lumen across the brush-border membrane (BBM) by a sodium-dependent transporter, SGLT, followed by exit across the basolateral membrane (BLM) via a facilitative, GLUT-mediated transporter. Our previous studies have shown that streptozotocin (STZ)-induced diabetes has no effect on SGLT-mediated transport but increases GLUTmediated transport across the renal BBM (Debnam et al. 1997), which is associated with increased levels of GLUT2, demonstrated by Western blotting. However, immunohistochemistry performed on unfixed tissue sections failed to detect this protein at the BBM. The present study investigated whether kidney fixation in vivo yielded the same pattern of GLUT2 expression as that obtained in unfixed tissue.