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The effect of systemic hypoxia on interstitial and blood adenosine, AMP, ADP and ATP in dog skeletal muscle
Author(s) -
Mo F. M.,
Ballard H. J.
Publication year - 2001
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2001.0593c.xd
Subject(s) - adenosine , hypoxia (environmental) , medicine , adenine nucleotide , endocrinology , pco2 , interstitial fluid , perfusion , arterial blood , chemistry , skeletal muscle , venous blood , adenosine diphosphate , nucleotide , oxygen , biochemistry , platelet , platelet aggregation , organic chemistry , gene
1 We investigated the effect of moderate systemic hypoxia on the arterial, venous and interstital concentration of adenosine and adenine nucleotides in the neurally and vascularly isolated, constant‐flow perfused gracilis muscles of anaesthetized dogs. 2 Systemic hypoxia reduced arterial P O2 from 129 to 28 mmHg, venous P O2 from 63 to 23 mmHg, arterial pH from 7.43 to 7.36 and venous pH from 7.38 to 7.32. Neither arterial nor venous P CO2 were changed. Arterial perfusion pressure remained at 109 ± 8 mmHg for the first 5 min of hypoxia, then increased to 131 ± 11 mmHg by 9 min, and then decreased again throughout the rest of the hypoxic period. 3 Arterial adenosine (427 ± 98 n m ) did not change during hypoxia, but venous adenosine increased from 350 ± 52 to 518 ± 107 n m . Interstitial adenosine concentration did not increase (339 ± 154 n m in normoxia and 262 ± 97 n m in hypoxia). Neither arterial nor venous nor interstitial concentrations of adenine nucleotides changed significantly in hypoxia. 4 Interstitial adenosine, AMP, ADP and ATP increased from 194 ± 40, 351 ± 19, 52 ± 7 and 113 ± 36 to 764 ± 140, 793 ± 119, 403 ± 67 and 574 ± 122 n m , respectively, during 2 Hz muscle contractions. 5 Adenosine, AMP, ADP and ATP infused into the arterial blood did not elevate the interstitial concentration until the arterial concentration exceeded 10 μ m . 6 We conclude that the increased adenosine in skeletal muscle during systemic hypoxia is formed by the vascular tissue or the blood cells, and that adenosine is formed intracellularly by these tissues. On the other hand, adenosine formation takes place extracellularly in the interstitial space during muscle contractions.