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Bradykinin evokes a Ca 2+ ‐activated chloride current in non‐neuronal cells isolated from neonatal rat dorsal root ganglia
Author(s) -
England S.,
Heblich Fay,
James I. F.,
Robbins J.,
Docherty R. J.
Publication year - 2001
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2001.0395k.x
Subject(s) - intracellular , extracellular , biophysics , bradykinin , chemistry , reversal potential , egta , medicine , endocrinology , bk channel , receptor , bradykinin receptor , membrane potential , patch clamp , biology , biochemistry , calcium
1 We have studied the effect of bradykinin (Bk) on fibroblast‐like satellite (FLS) cells isolated from cultures of neonatal rat dorsal root ganglia (DRG). 2 In voltage‐clamped FLS cells Bk evoked an inward current response that was concentration dependent with a half‐maximal concentration of 2 nM. 3 In indo‐1 AM‐loaded FLS cells Bk evoked a rise in intracellular Ca 2+ that was concentration dependent with a half‐maximal concentration of 1 nM. 4 The FLS cells still produced an inward current in response to Bk in the absence of extracellular Ca 2+ but the response was inhibited if the intracellular concentration of EGTA was increased from 0.5 to 5 mM, which suggests that the inward current was dependent on the release and subsequent rise of intracellular Ca 2+ . 5 The reversal potential of the Bk‐induced inward current was consistent with the current being due to an increase in Cl − conductance and shifted in a Nernstian manner when the intracellular Cl − concentration was reduced. 6 The inward current response to Bk was blocked by the B 2 receptor antagonist HOE‐140, which indicates that the response was due to activation of B 2 receptors. 7 The data suggest that Bk evokes a rise in intracellular Ca 2+ and activation of a Ca 2+ ‐activated Cl − conductance in the FLS cells and raise the possibility that FLS cells contribute to the pro‐inflammatory effects of Bk in DRG.