Premium
The paracellular component of water flow in the rat submandibular salivary gland
Author(s) -
Murakami Masataka,
ShacharHill Bruria,
Steward Martin C.,
Hill A. E.
Publication year - 2001
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2001.00899.x
Subject(s) - paracellular transport , submandibular gland , chemistry , radius , secretion , biophysics , salivary gland , endocrinology , medicine , biology , biochemistry , membrane , permeability (electromagnetism) , computer security , computer science
1 The pathway of water flow during salivary secretion by the isolated, perfused rat submandibular gland was examined using a family of homologous radiodextran molecules as probes of paracellular fluid transfer. 2 The secretion/perfusate ratio ( S/P ) of the secreted probes versus molecular radius during fluid secretion evoked by ACh could be resolved into two components: one that fitted a free‐diffusion (Stokes‐Einstein) curve and indicated diffusion through large channels, and a convective component that was linearly related to radius. 3 The convective component had a cut‐off point at 0.5 nm (5 Å) radius and an S/P intercept of near 1.0 at the radius of water, which indicates that most of the volume flow was paracellular. 4 The nature of such a paracellular flow is discussed together with the possible integration of this volume flow with the cellular transport of ions, resulting in an isotonic primary secretion from the gland.