Premium
Endothelin‐1‐endothelin receptor type A mediates closure of rat ductus arteriosus at birth
Author(s) -
Taniguchi Takanobu,
Azuma Hiroshi,
Okada Yuichi,
Naiki Hironobu,
Hollenberg Morley D.,
Muramatsu Ikunobu
Publication year - 2001
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2001.00579.x
Subject(s) - ductus arteriosus , antagonist , endocrinology , medicine , contraction (grammar) , endothelin 1 , endothelin receptor , in vivo , agonist , uterine contraction , receptor antagonist , receptor , chemistry , constriction , biology , uterus , microbiology and biotechnology
1 The ductus arteriosus (DA) undergoes rapid closure after birth as pulmonary circulation is established. The involvement of endothelin‐1 (ET1) in this closure mechanism is controversial. 2 The effect of ATZ1993 (ATZ), a non‐peptide antagonist for the ET A and ET B receptors, on postnatal closure and O 2 ‐induced contraction of the rat DA was investigated both in vivo and in vitro . Rat pups were delivered by Caesarean section and were given ATZ intraperitoneally. The minimum external DA diameter and the extent of DA constriction in vivo were evaluated at 2.5 h after birth. ATZ caused a dose‐dependent inhibition of DA closure in vivo . When rat pups were given ATZ at 2.5 h after birth, re‐opening of the DA was observed. 3 In vitro , ATZ also caused a marked inhibition of O 2 ‐induced and ET1‐induced DA contractions as did BQ123, an ET A ‐specific antagonist. In contrast, sarafotoxin S6c, an ET B ‐specific agonist, did not cause DA contraction and BQ788, an ET B ‐specific antagonist, did not affect O 2 ‐induced DA contraction. 4 In conclusion, ET1 and its cognate receptor ET A may play a physiological role in the postnatal closure of the rat DA in vivo.