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Electrically evoked neuropeptide release and neurogenic inflammation differ between rat and human skin
Author(s) -
Sauerstein Katja,
Klede Monika,
Hilliges Marita,
Schmelz Martin
Publication year - 2000
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2000.00803.x
Subject(s) - extravasation , calcitonin gene related peptide , neurogenic inflammation , neuropeptide , stimulation , microdialysis , substance p , endocrinology , medicine , chemistry , vasodilation , calcitonin , central nervous system , immunology , receptor
1 Protein extravasation and vasodilatation can be induced by neuropeptides released from nociceptive afferents (neurogenic inflammation). We measured electrically evoked neuropeptide release and concomitant protein extravasation in human and rat skin using intradermal microdialysis. 2 Plasmapheresis capillaries were inserted intradermally at a length of 1.5 cm in the volar forearm of human subjects or abdominal skin of rats. Capillaries were perfused with Ringer solution at a flow rate of 2.5 or 1.6 μl min −1 . After a baseline period of 60 min capillaries were stimulated electrically (1 Hz, 80 mA, 0.5 ms or 4 Hz, 30 mA, 0.5 ms) for 30 min using a surface electrode directly above the capillaries and a stainless‐steel wire inserted in the capillaries. Total protein concentration was assessed photometrically and calcitonin gene‐related peptide (CGRP) and substance P (SP) concentrations were measured by enzyme‐linked immunosorbent assay (ELISA). 3 In rat skin, electrical stimulation increased CGRP and total protein concentration in the dialysate. SP measurements showed a larger variance but only for the 1 Hz stimulation was the increased release significant. 4 In human skin, electrical stimulation provoked a large flare reaction and at a frequency of 4 Hz both CGRP and SP concentrations increased significantly. In spite of the large flare reactions no protein extravasation was induced, which suggests major species differences. 5 It will be of interest to investigate whether the lack of neurogenic protein extravasation is also valid under pathophysiological conditions.