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Regulation of volume‐activated chloride channels by P‐glycoprotein: phosphorylation has the final say!
Author(s) -
Idriss Haitham T.,
Hannun Yusuf A.,
Boulpaep Emile,
Basavappa Srisaila
Publication year - 2000
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2000.00629.x
Subject(s) - phosphorylation , chloride channel , chloride , chemistry , volume (thermodynamics) , glycoprotein , biophysics , microbiology and biotechnology , biochemistry , biology , thermodynamics , physics , organic chemistry
1 P‐glycoprotein (Pgp) is a transmembrane transporter causing efflux of a number of chemically unrelated drugs and is responsible for resistance to a variety of anticancer drugs during chemotherapy. 2 Pgp overexpression in cells is also associated with volume‐activated chloride channel activity; Pgp is thought to regulate such activity. 3 Reversible phosphorylation is a possible mechanism for regulating the transport and chloride channel regulation functions of Pgp. Protein kinase C (PKC) is a good candidate for inducing such phosphorylation. 4 Hierarchical multiple phosphorylation (e.g. of different serines and with different PKC isoforms) may shuttle the protein between its different states of activity (transport or channel regulation). Cell volume changes may trigger phosphorylation of Pgp at sites causing inhibition of transport. 5 The possible regulation of chloride channels by Pgp and the potential involvement of reversible phosphorylation in such regulation is reviewed.