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The nuclear chloride ion channel NCC27 is involved in regulation of the cell cycle
Author(s) -
Valenzuela Stella M.,
Mazzanti Michele,
Tonini Raffaella,
Qiu Min Ru,
Warton Kristina,
Musgrove Elizabeth A.,
Campbell Terence J.,
Breit Samuel N.
Publication year - 2000
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2000.00541.x
Subject(s) - chinese hamster ovary cell , ion channel , cell cycle , microbiology and biotechnology , chloride channel , cell , biology , chemistry , cell culture , biochemistry , genetics , receptor
1 NCC27 is a nuclear chloride ion channel, identified in the PMA‐activated U937 human monocyte cell line. NCC27 mRNA is expressed in virtually all cells and tissues and the gene encoding NCC27 is also highly conserved. Because of these factors, we have examined the hypothesis that NCC27 is involved in cell cycle regulation. 2 Electrophysiological studies in Chinese hamster ovary (CHO‐K1) cells indicated that NCC27 chloride conductance varied according to the stage of the cell cycle, being expressed only on the plasma membrane of cells in G2/M phase. 3 We also demonstrate that Cl − ion channel blockers known to block NCC27 led to arrest of CHO‐K1 cells in the G2/M stage of the cell cycle, the same stage at which this ion channel is selectively expressed on the plasma membrane. 4 These data strongly support the hypothesis that NCC27 is involved, in some as yet undetermined manner, in regulation of the cell cycle.