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Differential expression of volume‐regulated anion channels during cell cycle progression of human cervical cancer cells
Author(s) -
Shen MengRu,
Droogmans Guy,
Eggermont Jan,
Voets Thomas,
Ellory J. Clive,
Nilius Bernd
Publication year - 2000
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.2000.00385.x
Subject(s) - aphidicolin , cell cycle , cell growth , cancer cell , apoptosis , cell culture , cell cycle checkpoint , chemistry , cell cycle progression , cancer research , microbiology and biotechnology , cancer , biology , medicine , biochemistry , genetics
1 This study investigated the volume‐regulated anion channel (VRAC) of human cervical cancer SiHa cells under various culture conditions, testing the hypothesis that the progression of the cell cycle is accompanied by differential expression of VRAC activity. 2 Exponentially growing SiHa cells expressed VRACs, as indicated by the presence of large outwardly rectifying currents activated by hypotonic stress with the anion permeability sequence I − > Br − > Cl − . VRACs were potently inhibited by tamoxifen with an IC 50 of 4.6 μ m . 3 Fluorescence‐activated cell sorting (FACS) experiments showed that 59 ± 0.5, 5 ± 0.5 and 36 ± 1.1% of unsynchronized, exponentially growing cervical cancer SiHa cells were in G0/G1, S and G2/M stage, respectively. Treatment with aphidicolin (5 μ m ) arrested 88 ± 1.4% of cells at the G0/G1 stage. 4 Arrest of cell growth in the G0/G1 phase was accompanied by a significant decrease of VRAC activity. The normalized hypotonicity‐induced current decreased from 48 ± 5.2 pA pF −1 at +100 mV in unsynchronized cells to 15 ± 2.6 pA pF −1 at +100 mV in aphidicolin‐treated cells. After removal of aphidicolin, culturing in medium containing 10% fetal calf serum triggered a rapid re‐entry into the cell cycle and a concomitant recovery of VRAC density. 5 Pharmacological blockade of VRACs by tamoxifen or NPPB caused proliferating cervical cancer cells to arrest in the G0/G1 stage, suggesting that activity of this channel is critical for G1/S checkpoint progression. 6 This study provides new information on the functional significance of VRACs in the cell cycle clock of human cervical cancer cells.