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Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans
Author(s) -
Langberg Henning,
Skovgaard Dorthe,
Karamouzis Michael,
Bülow Jens,
Kjær Michael
Publication year - 1999
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.1999.919ab.x
Subject(s) - microdialysis , achilles tendon , isometric exercise , interstitial fluid , chemistry , ankle , medicine , blood flow , endocrinology , lipolysis , tendon , anesthesia , anatomy , adipose tissue , central nervous system
1 The metabolic processes that occur around the tendon during mechanical loading and exercise are undescribed in man. These processes are important for understanding the development of overuse inflammation and injury. 2 A microdialysis technique was used to determine interstitial concentrations of glycerol, glucose, lactate, prostaglandin E 2 (PGE 2 ) and thromboxane B 2 (TXB 2 ) as well as to calculate tissue substrate balance in the peritendinous region of the human Achilles tendon. Recovery of 48–62% (range) at rest and 70–77% during exercise were obtained for glycerol, glucose and PGE 2 . 3 Six young healthy humans were studied at rest, during 30 min of intermittent static plantar flexion of the ankle at a workload corresponding to individual body weight, and during 60 min of recovery. Microdialysis was performed in both legs with simultaneous determination of blood flow by 133 Xe washout in the same area, and blood sampling from the radial artery. 4 With exercise, the net release of lactate as well as of glycerol from the peritendinous space of the Achilles tendon increased 2‐fold ( P < 0.05 ). Furthermore a 100% increase in interstitial concentration of PGE 2 and TXB 2 was found, but it was only significant for TXB 2 ( P < 0.05 ). As peritendinous blood flow increased 2‐ to 3‐fold during intermittent static contractions, this indicates also that the output of these substances from the tissue increased during exercise. 5 This study indicates that both lipid and carbohydrate metabolism as well as inflammatory activity is accelerated in the peritendinous region of the human Achilles tendon with dynamic loading.