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Pulsatile shear stress leads to DNA fragmentation in human SH‐SY5Y neuroblastoma cell line
Author(s) -
Triyoso Dina H.,
Good Theresa A.
Publication year - 1999
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.1999.355ac.x
Subject(s) - dna fragmentation , fragmentation (computing) , sh sy5y , pulsatile flow , nitric oxide , shear stress , programmed cell death , apoptosis , chemistry , biophysics , cell culture , microbiology and biotechnology , biology , materials science , biochemistry , neuroblastoma , endocrinology , ecology , genetics , composite material , organic chemistry
1 Using an in vitro model of shear stress‐induced cell injury we demonstrate that application of shear to differentiated human SH‐SY5Y cells leads to cell death characterized by DNA fragmentation. Controlled shear stress was applied to cells via a modified cone and plate viscometer. 2 We show that pulsatile shear stress leads to DNA fragmentation, as determined via flow cytometry of fluorescein‐12‐dUTP nick‐end labelled cells, in 45 ± 4% of cells. No lactate dehydrogenase (LDH) release was observed immediately after injury; however, 24 h after injury significant LDH release was observed. 3 Nitric oxide production by cells subjected to pulsatile shear increased two‐ to threefold over that in unsheared control cells. 4 Inhibition of protein synthesis, nitric oxide production, Ca 2+ entry into cells, and pertussis toxin‐sensitive G protein activation attenuated the shear stress‐induced cell injury. 5 Our results show for the first time that application of pulsatile shear stress to a neuron‐like cell in vitro leads to nitric oxide‐dependent cell death.