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Hexose transport in the apical and basolateral membranes of enterocytes in chickens adapted to high and low NaCl intakes
Author(s) -
Garriga Carles,
Moretó Miquel,
Planas Joana M.
Publication year - 1999
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.1999.189af.x
Subject(s) - ileum , phlorizin , brush border , jejunum , cytochalasin b , hexose , apical membrane , biology , population , epithelial polarity , biochemistry , chemistry , glucose transporter , membrane , medicine , biophysics , endocrinology , cell , enzyme , vesicle , environmental health , insulin
1 The effect of a low‐NaCl diet (LS diet) on the properties of hexose transport across the brush‐border and basolateral membranes of enterocytes from jejunum, ileum and rectum of the chicken was investigated. 2 In the brush‐border membrane, LS adaptation had no effect on K m for α‐methyl‐ d ‐glucoside while V max values were significantly reduced in the ileum and in the rectum. All Scatchard plots of specific [ 3 H]phlorizin binding give a straight line, consistent with a single population of binding sites. Phlorizin binding vs. α‐methyl‐ d ‐glucoside maximal transport rates showed a linear correlation. 3 In the basolateral membrane, the LS diet did not modify the K m for d ‐glucose but reduced the V max in the ileum and in the rectum. Scatchard plots of [ 3 H]cytochalasin B binding support the view that there is a single transport system in this membrane. There was a linear correlation between cytochalasin B binding and d ‐glucose V max values. 4 The response of the chicken intestine to LS intake consists of a dramatic reduction in the number of glucose transporters in both apical and basolateral membranes of the rectum, an intermediate response in the ileum and no significant effects in the jejunum.