Premium
Cloning and functional expression of rat ether‐à‐go‐go ‐like K + channel genes
Author(s) -
Engeland Birgit,
Neu Axel,
Ludwig Jost,
Roeper Jochen,
Pongs Olaf
Publication year - 1998
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.1998.647ba.x
Subject(s) - herg , chinese hamster ovary cell , potassium channel , microbiology and biotechnology , complementary dna , tetraethylammonium , chemistry , potassium channel blocker , biology , biophysics , biochemistry , gene , potassium , receptor , organic chemistry
1 Screening of rat cortex cDNA resulted in cloning of two complete and one partial orthologue of the Drosophila ether‐à‐go‐go‐ like K + channel ( elk).2 Northern blot and reverse transcriptase‐polymerase chain reaction (RT‐PCR) analysis revealed predominant expression of rat elk mRNAs in brain. Each rat elk mRNA showed a distinct, but overlapping expression pattern in different rat brain areas. 3 Transient transfection of Chinese hamster ovary (CHO) cells with rat elk1 or rat elk2 cDNA gave rise to voltage‐activated K + channels with novel properties. 4 RELK1 channels mediated slowly activating sustained potassium currents. The threshold for activation was at −90 mV. Currents were insensitive to tetraethylammonium (TEA) and 4‐aminopyridine (4‐AP), but were blocked by micromolar concentrations of Ba 2+ . RELK1 activation kinetics were not dependent on prepulse potential like REAG‐mediated currents. 5 RELK2 channels produced currents with a fast inactivation component and HERG‐like tail currents. RELK2 currents were not sensitive to the HERG channel blocker E4031.