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Fibre type‐specific gene expression activated by chronic electrical stimulation of adult mouse skeletal muscle fibres in culture
Author(s) -
Liu Yewei,
Schneider Martin F.
Publication year - 1998
Publication title -
the journal of physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.802
H-Index - 240
eISSN - 1469-7793
pISSN - 0022-3751
DOI - 10.1111/j.1469-7793.1998.337be.x
Subject(s) - stimulation , myosin , skeletal muscle fibers , skeletal muscle , messenger rna , actin , gene expression , in vitro , isometric exercise , chemistry , biology , microbiology and biotechnology , endocrinology , medicine , gene , biochemistry
1 Fast‐twitch skeletal muscle fibres were enzymatically dissociated from adult mouse flexor digitorum brevis (FDB) muscles and maintained in culture without or with chronic low frequency stimulation (one 5 s train of 5 Hz pulses per minute) for up to 6 days. Single fibre reverse transcriptase‐polymerase chain reaction was conducted to coamplify β‐myosin heavy chain (β‐MHC) and α‐skeletal actin mRNA from the same fibre. 2 Chronic low frequency electrical stimulation of FDB fibres in culture increased the level of mRNA for β‐MHC. In unstimulated fibres there was a slight decline in the β‐MHC mRNA level. As an internal control there was no increase in the level of mRNA for α‐actin in the identical individual stimulated or unstimulated fibres. 3 Neither the percentage of fibres exhibiting β‐MHC protein nor the Ca 2+ transients recorded from individual fibres subjected to the same pattern of stimulation showed any difference between stimulated and unstimulated fibres over the period in culture. 4 This system provides a convenient in vitro model system for studying activity‐dependent control of fibre type‐specific gene expression in adult skeletal muscle fibres in culture.