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Expression of Ten‐m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice
Author(s) -
Murakami Takashi,
Fukunaga Tomohiro,
Takeshita Nobuo,
Hiratsuka Koichi,
Abiko Yoshimitsu,
Yamashiro Takashi,
TakanoYamamoto Teruko
Publication year - 2010
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1111/j.1469-7580.2010.01267.x
Subject(s) - cartilage , chondrogenesis , chondrocyte , biology , in situ hybridization , microbiology and biotechnology , type ii collagen , mesenchymal stem cell , gene expression , anatomy , gene , genetics
It has been speculated that the mandibular condyle develops via the differentiation of the fibroblast‐like cells covering the condyle into chondrocytes; however, the developmental mechanisms behind this process have not been revealed. We used laser‐capture microdissection and cDNA microarray analysis to elucidate the genes that are highly expressed in these fibroblast‐like cells. Among these genes, the transcription of Ten‐m/Odz3 was significantly increased in the fibroblast‐like cells compared with other cartilage tissues. For the first time, we describe the temporal and spatial expression of Ten‐m/Odz3 mRNA in relation to the expression of type I , II , and X collagen mRNA , as determined by in‐situ hybridization in mouse mandibular condylar cartilage and mouse femoral cartilage during the early stages of development. Ten‐m/Odz3 was expressed in the fibrous layer and the proliferating and mature chondrocyte layers, which expressed type I and II collagen, respectively, but was not detected in the hypertrophic chondrocyte layer. Furthermore, we evaluated the in‐vitro expression of Ten‐m/Odz3 using ATDC5 cells, a mouse chondrogenic cell line. Ten‐m/Odz3 was expressed during the early stage of the differentiation of mesenchymal cells into chondrocytes. These findings suggest that Ten‐m/Odz3 is involved in the differentiation of chondrocytes and that it acts as a regulatory factor in the early stages of the development of mandibular condylar cartilage.