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Micro‐magnetic resonance imaging of avian embryos
Author(s) -
Li Xiaojing,
Liu Jia,
Davey Megan,
Duce Suzanne,
Jaberi Neema,
Liu Gang,
Davidson Gemma,
Tenent Seaneen,
Mahood Ruth,
Brown Phoebe,
Cunningham Craig,
Bain Andrew,
Beattie Kevin,
McDonald Laura,
Schmidt Katy,
Towers Matthew,
Tickle Cheryll,
Chudek Sandy
Publication year - 2007
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1111/j.1469-7580.2007.00825.x
Subject(s) - quail , magnetic resonance imaging , embryo , organogenesis , biology , anatomy , focus (optics) , microbiology and biotechnology , medicine , physics , gene , radiology , genetics , endocrinology , optics
Chick embryos are useful models for probing developmental mechanisms including those involved in organogenesis. In addition to classic embryological manipulations, it is possible to test the function of molecules and genes while the embryo remains within the egg. Here we define conditions for imaging chick embryo anatomy and for visualising living quail embryos. We focus on the developing limb and describe how different tissues can be imaged using micro‐magnetic resonance imaging and this information then synthesised, using a three‐dimensional visualisation package, into detailed anatomy. We illustrate the potential for micro‐magnetic resonance imaging to analyse phenotypic changes following chick limb manipulation. The work with the living quail embryos lays the foundations for using micro‐magnetic resonance imaging as an experimental tool to follow the consequences of such manipulations over time.