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Tissue‐plastinated vs. celloidin‐embedded large serial sections in video, analog and digital photographic on‐screen reproduction: a preliminary step to exact virtual 3D modelling, exemplified in the normal midface and cleft‐lip and palate
Author(s) -
Landes Constantin A.,
Weichert Frank,
Geis Philipp,
Wernstedt Katrin,
Wilde Anja,
Fritsch Helga,
Wagner Mathias
Publication year - 2005
Publication title -
journal of anatomy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 118
eISSN - 1469-7580
pISSN - 0021-8782
DOI - 10.1111/j.1469-7580.2005.00438.x
Subject(s) - photography , haematoxylin , anatomy , microtome , materials science , biomedical engineering , medicine , pathology , art , staining , visual arts
This study analyses tissue‐plastinated vs. celloidin‐embedded large serial sections, their inherent artefacts and aptitude with common video, analog or digital photographic on‐screen reproduction. Subsequent virtual 3D microanatomical reconstruction will increase our knowledge of normal and pathological microanatomy for cleft‐lip‐palate (clp) reconstructive surgery. Of 18 fetal (six clp, 12 control) specimens, six randomized specimens (two clp) were BiodurE12 ‐ plastinated, sawn, burnished 90 µm thick transversely (five) or frontally (one), stained with azureII/methylene blue, and counterstained with basic‐fuchsin (TP‐AMF). Twelve remaining specimens (four clp) were celloidin‐embedded, microtome‐sectioned 75 µm thick transversely (ten) or frontally (two), and stained with haematoxylin–eosin (CE‐HE). Computed‐planimetry gauged artefacts, structure differentiation was compared with light microscopy on video, analog and digital photography. Total artefact was 0.9% (TP‐AMF) and 2.1% (CE‐HE); TP‐AMF showed higher colour contrast, gamut and luminance, and CE‐HE more red contrast, saturation and hue ( P < 0.4). All (100%) structures of interest were light microscopically discerned, 83% on video, 76% on analog photography and 98% in digital photography. Computed image analysis assessed the greatest colour contrast, gamut, luminance and saturation on video; the most detailed, colour‐balanced and sharpest images were obatined with digital photography ( P < 0.02). TP‐AMF retained spatial oversight, covered the entire area of interest and should be combined in different specimens with CE‐HE which enables more refined muscle fibre reproduction. Digital photography is preferred for on‐screen analysis.