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Response of Isolated Ruminant Mammary Arteries to the Long R 3 Analogue of Insulin‐Like Growth Factor I
Author(s) -
Gow I. F.
Publication year - 2000
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1111/j.1469-445x.2000.01973.x
Subject(s) - sodium nitroprusside , endocrinology , medicine , acetylcholine , insulin like growth factor , endothelium , blood vessel , mammary artery , insulin , nitric oxide , vasodilation , chemistry , biology , growth factor , artery , receptor
Isolated mammary arteries from ruminants were used in a conventional organ bath system. Acetylcholine relaxed bovine but not ovine mammary arteries; both types responded to sodium nitroprusside. Noradrenaline (NA) caused a dose‐dependent increase in generated tension. An analogue of insulin‐like growth factor I (long R 3 ‐IGF‐I) caused a rightward shift in the NA response curve in bovine vessels with intact endothelium ( P < 0.02), and also in sheep arteries ( P < 0.01). In bovine vessels, this effect was abolished when the endothelium was removed. The effect of long R 3 ‐IGF‐I in bovine vessels was abolished by N ω ‐nitro‐L‐arginine methyl ester (L‐NAME) an inhibitor of nitric oxide synthase, suggesting the effect of IGF‐I on mammary arteries in vitro requires NO generation.